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KMID : 0604520080340020101
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Journal of the Society of Cosmetic Scientists of Korea
2008 Volume.34 No. 2 p.101 ~ p.107
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Whitening Effect of Angelica keiskei Koidzumi Extract by Inhibition of Endothelin-1 Production and Melanogenesis
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Park Sun-Hee
Lee Bang-Yong
Han Chang-Sung
Kim Jin-Guk
Kim Kyoung-Tae
Kim Ki-Ho
Kim Young-Heui
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Abstract
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In order to investigate the potential of a Angelica keiskei Koidzumi extract and its fractions as an active ingredient for whitening cosmetics, we prepared Angelica keiskei Koidzumi extract(70% ethanol), and the aqueous suspension was successively extracted with hexane, ethyl acetate(EA), and n-butanol fraction. We measured their inhibitory effects on mushroom tyrosinase and melanin synthesis in B16 melanoma cells and normal human kerationocytes in vitro. They did not show inhibitory activity against mushroom tyrosinase and the melanin synthesis except hexane and EA fractions. Hexane and EA fractions markedly inhibited cellular tyrosinase activity at a lower concentration(25 and 5 , respectively) than arbutin(250 ). We also quantified the released amount of endothelin-1(ET-1), a mitogen of melanocyte, and , a mediator of UVB-induced inflammation. Hexane and EA fractions did not affect production, but they decreased UVB-induced ET-1 production in normal human keratinocytes in a dose dependant manner. As a result, hexane and EA fractions could inhibit the melanogenesis through the inhibition of UVB-induced ET-1 production in normal human keratinocytes. This result suggests that hexane and EA fractions of Angelica keiskei Koidzumi extract could be used as an active ingredient for cosmetics.
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KEYWORD
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Angelica keiskei Koidzumi, melanogenesis, endothelin-1, tyrosinase
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